The IDH3F3A kit is a ready-to-use multiplex test for the detection and quantification by digital PCR of mutations in the H3F3A (H3 Histone Family Member 3A) gene.
Distinction of G34R, G34V, and K27M mutations of the H3F3A gene by liquid biopsy
The IDH3F3A kit is a ready-to-use multiplex test for the detection and quantification by digital PCR of mutations in the H3F3A (H3 Histone Family Member 3A) gene.
This optimized reaction system offers a multiplexed solution for simultaneous amplification of H3F3A mutations at positions K27M, G34R and G34V in a single PCR reaction for each sample.
The kit can be used oncirculating free DNA (cf-DNA) samples extracted from plasma, or on genomic DNA (gDNA) samples isolated from formalin-fixed, paraffin-embedded (FFPE) tissue.
Sample
Circulating free DNA (cf-DNA) extracted from plasma or genomic DNA (gDNA) isolated from formalin-fixed, paraffin-embedded tissue (FFPE)
Targets
G34R
G34V
K27M
Controls
Target Positive Control included in the kit
LoB (Plasma)
Bio-Rad: G34R/V: 2 droplets | K27M: 1 droplet
Stilla: G34R/V: 3 droplet crystals | K27M: 3 droplet crystals
Turnaround time
Bio-Rad: 4h (96 tests)
Stilla: 3h (12 tests)
dPCR platforms
Bio-Rad: QX200™ Droplet Digital™ PCR System
Stilla: 3-color naica® system with Sapphire chips
Storage T°C
From -26°C to -16°C
Reference
IDH3F3A(b)-25
IDH3F3A(s)-25
Certification