The IDEGFR-SENSI kit is a ready-to-use multiplex assay for digital PCR detection of common activating mutations (L858R, L861Q and exon 19 deletions) in theEGFR (Epidermal Growth Factor Receptor) gene.
Distinction of sensitive mutations L858R, L861Q, and Del19 of the EGFR gene by liquid biopsy
The IDEGFR-SENSI kit is a ready-to-use multiplex assay for digital PCR detection of common activating mutations (L858R, L861Q and exon 19 deletions) in theEGFR (Epidermal Growth Factor Receptor) gene.
This optimized reaction system offers a multiplexed solution enabling simultaneous amplification in a single PCR reaction ofEGFR mutations in each sample, including deletions inexon 19 and two substitutions, L858R and L861Q, in exon 21.
This IDEGFR-SENSI kit can be used in combination with the IDEGFR-RESIST and IDEGFR-RARE products.
The kit can be used on circulating DNA samples (cf-DNA) extracted from plasma, or on genomic DNA samples (gDNA) isolated from formalin-fixed, paraffin-embedded (FFPE) tissue.
Sample
Circulating free DNA (cf-DNA) extracted from plasma or genomic DNA (gDNA) isolated from formalin-fixed, paraffin-embedded tissue (FFPE)
Targets
L861Q, L858R and Del19
Controls
Target Positive Control Included in the kit
LoB (Plasma)
BioRad: L861Q: 1 droplet | L858R-Del19: 1 droplet
Stilla: L861Q: 6 droplet crystals | L858R: 1 droplet crystal | Del19: 1 droplet crystal
Turnaround time
Bio-Rad: 4h (96 tests)
Stilla: 3h (12 tests)
dPCR platforms
Bio-Rad: QX200™ Droplet Digital™ PCR System
Stilla: 3-color naica® system with Sapphire chips
Storage T°C
From -26°C to -16°C
Reference
IDEGFR(b)SENSI-V3-50
IDEGFR(s)SENSI-50
Certification