The IDEGFR-RARE kit is a ready-to-use multiplex test for digital PCR detection of activating or resistance mutations (G719X, Insertions exon 20 and S768I) in theEGFR (Epidermal Growth Factor Receptor) gene.
Distinction of resistance mutations T790M and C797S in Ex20 of the EGFR gene by liquid biopsy
The IDEGFR-RARE kit is a ready-to-use multiplex test for digital PCR detection of activating or resistance mutations (G719X, Insertions exon 20 and S768I) in theEGFR (Epidermal Growth Factor Receptor) gene.
This optimized reaction system offers a multiplexed solution for the simultaneous amplification of EGFR mutations, including G719X, S768I and exon 20 insertions, in a single PCR reaction for each sample.
This IDEGFR-RARE kit can be used in combination with the IDEGFR-SENSI and IDEGFR-RESIST products.
The kit can be used on circulating DNA samples (cf-DNA) extracted from plasma or on genomic DNA samples (gDNA) isolated from formalin-fixed, paraffin-embedded (FFPE) tissue.
Sample
Circulating free DNA (cf-DNA) extracted from plasma or genomic DNA (gDNA) isolated from formalin-fixed, paraffin-embedded tissue (FFPE)
Targets
G719X in Ex18 & Ins20/S768I in Ex20
Controls
Target Positive Control included in the kit
LoB (Plasma)
Bio-Rad: G719X: 3 droplets | Exon 20: 2 droplets
Stilla: G719X: 3 droplet crystals | Ins20: 3 droplet crystals | S768I: 1 droplet crystal
Turnaround time
Bio-Rad: 4h (96 tests)
Stilla: 3h (12 tests)
dPCR platforms
Bio-Rad: QX200™ Droplet Digital™ PCR System
Stilla: 3-color naica® system with Sapphire chips
Storage T°C
From -26°C to -16°C
Reference
IDEGFR(b)RARE-50
IDEGFR(s)RARE-50
Certification