Distinction of G34R, G34V, and K27M mutations of the H3F3A gene by liquid biopsy
The IDH3F3A kit is a ready-to-use multiplex test for the detection and quantification by digital PCR of mutations in the H3F3A (H3 Histone Family Member 3A) gene.
This optimized reaction system offers a multiplexed solution for simultaneous amplification of H3F3A mutations at positions K27M, G34R and G34V in a single PCR reaction for each sample.
The kit can be used oncirculating free DNA (cf-DNA) samples extracted from plasma, or on genomic DNA (gDNA) samples isolated from formalin-fixed, paraffin-embedded (FFPE) tissue.
Sample
Circulating free DNA (cf-DNA) extracted from plasma or genomic DNA (gDNA) isolated from formalin-fixed, paraffin-embedded tissue (FFPE)
Targets
G34R
G34V
K27M
Controls
Target Positive Control included in the kit
LoB (Plasma)
Bio-Rad: G34R/V: 2 droplets | K27M: 1 droplet
Stilla: G34R/V: 3 droplet crystals | K27M: 3 droplet crystals
Turnaround time
Bio-Rad: 4h (96 tests)
Stilla: 3h (12 tests)
dPCR platforms
Bio-Rad: QX200™ Droplet Digital™ PCR System
Stilla: 3-color naica® system with Sapphire chips
Storage T°C
From -26°C to -16°C
Reference
IDH3F3A(b)-25
IDH3F3A(s)-25
Certification
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