Distinction of E542K, E545K, Q546K, H1047R, and H1047 mutations of the PIK3CA gene by liquid biopsy
The IDPIK3CA kit is a ready-to-use multiplex test for the detection and quantification by digital PCR of mutations in the PIK3CA (phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha) gene.
This optimized reaction system offers a multiplexed solution enabling simultaneous amplification of PIK3CA mutations at positions E542K, E545K, Q546K, H1047R and H1047L for each sample in a single PCR reaction .
The kit can be used on circulating DNA samples (cf-DNA) extracted from plasma, or on genomic DNA samples (gDNA) isolated from formalin-fixed, paraffin-embedded (FFPE) tissue.
Sample
Circulating free DNA (cf-DNA) extracted from plasma or genomic DNA (gDNA) isolated from formalin-fixed, paraffin-embedded tissue (FFPE)
Targets
Exon 9: E542K, E545K, Q546K
Exon 20: H1047R, H1047L
Controls
Target Positive Control included in the kit
LoB (Plasma)
Bio-Rad: Ex9 mut: 3 droplets | Ex20 mut: 3 droplets
Stilla: Ex9 mut: 4 droplet crystals | Ex20 mut: 2 droplet crystals
Turnaround time
Bio-Rad: 4h (96 tests)
Stilla: 3h (12 tests)
dPCR platforms
Bio-Rad: QX200™ Droplet Digital™ PCR System
Stilla: 3-color naica® system with Sapphire chips
Storage T°C
From -26°C to -16°C
Reference
IDPIK3CA(b)-25
IDPIK3CA(s)-25
Certification
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