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Logo IDsolutions Contact Montpellier
Jobs
Documentation area
Contact
  • English
    • French
  • English
    • French
  • English
    • French
  • English
    • French
  • About us
  • Expertise
    Technology
    Liquid Biopsy
    Métrologie et Maintenance
  • Oncology
    DNA qualification
    dPCR Reagents
    Extraction solutions
    IDQUANT
    Quantification and standardization
    IDENTIFY
    Research tests
    IDNAPTEX
    Probes and primers
    Kits
    Automates
    Accessories
    IDXTRACT
    IDXTRACT-MAG
    IDXTRACT-MAG-FFPE
    IDEAL 96 High Purity Extraction Robot
    IDEAL 32 Extraction Robot
    IDWELL96
    IDELU96
    IDTIP96
    IDWELL32
    IDTIP32
    IDLYSE24
  • Infectiology
    Diagnostic devices
    qPCR Reagents
    Extraction solutions
    Respiratory infections
    Gastrointestinal infections
    MonkeyPox virus
    Kit
    ID™ SARS-CoV-2/Influenza Triplex
    iSolutions™ Essential Enteric Bacteria Fullplex qPCR
    iSolutions™ Enteric Bacteria & C. difficile Fullplex qPCR
    iSolutions™ Enteric Virus Fullplex qPCR
    iSolutions™ MonkeyPox Fullplex qPCR
    MAG FAST Extraction Kit
    ID™ One-Step LysMix
    Microbial Lysis Solutions
    Anti-inhibitor Elution Buffer
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IDNAPTEX H3F3A

Set of probes and primers for detecting
the H3F3A mutation

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Ready-to-use
gDNA & cfDNA
Patented technology

Product description

IDNAPTEX H3F3A reliably identifies H3F3A mutations. It uses an optimised mix of primers and fluorescent probes.

IDNAPTEX H3F3A targets Exon 1 mutations (K27M, G34R, G34V).

ICE* technology ensures optimal sensitivity and reproducibility. Developed by ID SOLUTIONS, it is essential for the analysis of plasma (cfDNA) or FFPE tissue (gDNA) samples.

The reagents are supplied in pre-mixed tubes. The primers and probes are ready to use to simplify preparation. This configuration improves the reproducibility of results.

*Not included in the kit

Target Positive Control (TPC) on Bio-Rad platform:

Including the DIgestion Control Efficiency (DICE)

Target Positive Control mimicking a non-methylated sample

The DICE allows the population positioning to discriminate digested control vs non digested control and the calculation of digestion efficiency.

MLH1 population represents the non methylated MLH1 population.

Profil obtained from a FFPE patient sample on Bio-Rad platform:

10% methylated patient sample

DICE-M and DICE calculation shows a digestion efficiency of 99%

The ratio of methylated MLH1 promoter represents 10%

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Features

Sample

Circulating free DNA (cfDNA) extracted from plasma samples

Genomic DNA (gDNA) isolated from formalin-fixed, paraffin-embedded (FFPE) tissue

Targets

Exon 1 : K27M, G34R, G34V

Controls

Target Positive Control (TPC) included in the kit DIgestion Control Efficiency (DICE)

LoB (95%)

MLH1-M: 3% (Methylation percentage)

LoD (95%)

MLH1-M: 5% (Methylation percentage)

Turnaround time

24 samples: < 3 hours (from DNA to results)

dPCR Platforms

Bio-Rad : QX200™ Droplet Digital™ PCR System

Storage T°C

-20°C

Reference

IDmMLH1(b)-25

Certification

Research Use Only (RUO)

Courbes d'amplification

Associated products

IDXtract

IDXTRACT-50

IDXtract-MAG

IDXTRACT-MAG-24/100

IDXtract-MAG-FFPE

IDXTRACT-MAG-FFPE-96/384

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