Distinction of L597X, A598X, V600X, and K601E mutations of the BRAF gene by liquid biopsy
The IDBRAF kit is a ready-to-use multiplex test for the detection and quantification by digital PCR of exon 15 mutations in the BRAF (B-Rad proto-oncogene, serine/threonine kinase) gene.
This optimized reaction system offers a multiplexed solution enabling simultaneous amplification in a single PCR reaction of BRAF mutations at positions L597X, A598X, V600X and K601E, with a distinction for the V600E mutation.
The kit can be used on circulating DNA samples (cf-DNA) extracted from plasma, or on genomic DNA samples (gDNA) isolated from formalin-fixed, paraffin-embedded (FFPE) tissue.
Sample
Circulating free DNA (cf-DNA) extracted from plasma or genomic DNA (gDNA) isolated from formalin-fixed, paraffin-embedded tissue (FFPE)
Targets
BRAF mutations: L597, A598, V600 and K601, with a distinction of the V600E mutation
Controls
Target Positive Control included in the kit
LoB (Plasma)
Bio-Rad: BRAF V600E: 1 droplet | BRAF w/o V600E: 3 droplets
Stilla: BRAF V600E: 1 droplet crystal | BRAF w/o V600E: 3 droplet crystals
Turnaround time
Bio-Rad: 4h (96 tests)
Stilla: 3h (12 tests)
dPCR platforms
Bio-Rad: QX200™ Droplet Digital™ PCR System
Stilla: 3-color naica® system with Sapphire chips
Storage T°C
From -26°C to -16°C
Reference
IDBRAF(b)-50
IDBRAF(s)-50
Certification
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