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IDNAPTEX KRAS 3X

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IDNAPTEX KRAS 3X

Set of probes and primers for the detection
of KRAS mutations in exons 2, 3 and 4

Optimized for

QIAcuity® Digital PCR system

Product description

IDNAPTEX KRAS 3X enables the reliable and differentiated detection of KRAS gene mutations, as well as the identification of the wild-type allele, thanks to an optimised mix of primers and fluorescent probes, specifically designed for digital PCR (dPCR) analysis.

The test provides comprehensive and optimised coverage of mutated regions:

  • exon 2: G12C, G12D, G12V, G12R, G12S, G12A, G12F, G13C, G13D, G13R, G13S
  • exon 3: Q61R, Q61L, Q61E, Q61H, Q61K, Q61P
  • exon 4: A146T, A146P, A146V, K117N

Based on ICE* technology developed by ID SOLUTIONS, IDNAPTEX KRAS 3X guarantees high sensitivity and excellent reproducibility, including on challenging clinical matrices such as plasma (cfDNA) or FFPE tissue (gDNA).

The reagents are supplied in pre-mixed tubes. They contain ready-to-use primers and probes. This simplifies preparation and minimises handling. It also ensures optimal standardisation of results in routine laboratory work.

*Not included in the kit

Ready to use
gDNA & cfDNA
ID SOLUTIONS’ patented* technology

Performance

Detection of KRAS Variants in Exons 2, 3 and 4 from Liquid Biopsy and Tissue Samples Using IDNAPTEX Assays on QIAcuity

DOWNLOAD (PDF)

The ICE control has been implemented specifically to validate the extraction procedure for each sample tested.

Features

Content

4 tubes of PRIMEX KRAS Ex2/3 primers and probes – 24 reactions each
4 tubes of PRIMEX KRAS Ex4 primers and probes – 24 reactions each
2 tubes of TPC KRAS 3X target process controls – 10 reactions each

Samples

Circulating free DNA (cfDNA) extracted from a plasma sample

Genomic DNA (gDNA) Isolated form formalin-fixed, paraffin-embedded tissue (FFPE)

Targets

PRIMEX KRAS Ex2/3
Exon 2 : G12C, G12D, G12V, G12R, G12S, G12A, G12F, G13C, G13D, G13R, G13S
Exon 3 : Q61R, Q61L, Q61E, Q61H, Q61K, Q61P

PRIMEX KRAS Ex4
Exon 4 : A146T, A146P, A146V, K117N

Controls

Target Positive Control (TPC) included in the kit DIgestion Control Efficiency (DICE)

Turnaround time

24 samples: < 3h (DNA to product)

Platform

QIAcuity® 5Plex dPCR system
QIAcuity® Probe PCR kit
QIAcuity® Nanoplate 26K

Storage T°C

-20°C

Reference

1141-011107-96

Associated product

ID-ICE (ref. 1171-000000-96)

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*ID-ICE can be ordered separately. The patented ID SOLUTIONS technology is protected in Europe by patent number EP 3565902 and in the United States by patent number 12049664.

QIAcuity is a registered trademark of QIAGEN. QIAcuity dPCR instruments, consumables and tests are sold by QIAGEN under licence from Bio-Rad Laboratories, Inc. for use on QIAcuity Digital PCR instruments and exclude rights for use in paediatric applications.

QIAcuity is intended for molecular biology applications. This product is not intended for use in the diagnosis, prevention, or treatment of disease. Therefore, the performance characteristics of the product for clinical use (i.e., diagnostic, prognostic, therapeutic, or blood banking) are unknown.

Trademarks: QIAGEN®, QIAcuity®. Registered names, trademarks, etc. used in this document, even if not specifically marked as such, may be protected by law.

ID SOLUTIONS and IDNAPTEX are registered trademarks of ID SOLUTIONS.

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IDXtract-MAG

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IDXtract-MAG-FFPE

IDXTRACT-MAG-FFPE-96/384