IDXtract-MAG
IDXtract-MAG is a total circulating DNA extraction kit (cDNA) optimized for use on biological samples such as blood plasma.
The kit is based on magnetic bead extraction, allowing reproducible recovery of high quality cDNA. It contains a calibrated Positive Control (TPC) and an Internal Extraction Control (ICE) allowing the qualification and the quantification of the extraction process and the standardization of the concentration for each sample.
This kit concentrates the total cDNA in a small elution volume suitable for a wide range of applications, including real-time PCR, digital PCR (dPCR), next-generation sequencing (NGS) and genomic hybridization technologies.
The extraction can either be done manually using suitable magnetic racks, or carried out by an automate such as IDEAL (ID-Solutions) or KingFisher Flex (ThermoFisher).
“Method for the detection and quantification of circulating DNA and uses” PATENTED TECHNOLOGY
Official publication numbers of applications “FR3061720” / “WO2018/127674”
| Technology | magnetic beads |
| Product references and formats | IDXtract-MAG-24 (24 preps of 4ml) IDXtract-MAG-100 (100 preps of 4ml) |
| Matrix | plasma from 1 to 8 ml |
| Utilisation | automated protocol |
| Applications | NGS (Next-Generation Sequencing), dPCR (digital PCR), Real-Time PCR |
| Duration of protocol | <2h for 24 preps |
| Storage temperature | ambient temperature |
| Stability | 1 year |
Associated consumables
- IDWELL-32, Deepwell plate 2mL (96 wells)
- IDTIP-32, Magnetic rod’s tip (8 samples)
- IDWELL 24, Deepwell plate 10mL (24 wells)
- IDTIPWELL-24, Combi Tip comb/Deepwell 10mL (24 tips/wells)
Associated materials
Fig.1
Characteristics of IDXTRACT-MAG extraction kit.
Fig.2
Example of the cDNA profile obtained after extraction of healthy plasma with the IDXtract kit.
Documentation on request to info@id-solutions.fr
