IDXtract-MAG
a cfDNA extraction kit using magnetic beads method
IDXtract-MAG, an extraction kit integrating a calibrated positive control (TPC) and an internal extraction control (ICE), which allows the validation of each analytical step.
IDXtract-MAG is a circulating cell-free DNA (cfDNA) extraction kit optimized for use on biological samples, such as blood plasma.
This kit contains a calibrated positive control (TPC) and an internal extraction control (ICE), allowing for qualification and quantification of the extraction process and standardization of the samples` concentration. The extraction kit is magnetic beads-based system allows high-quality cfDNA recovery in a reproducible manner.
This kit concentrates the total cfDNA in a low elution volume suitable for a wide range of applications, including real-time PCR, digital PCR (dPCR), Next-Generation Sequencing (NGS) and genomic hybridization technologies.
The extraction can be performed by a robot, such as IDEAL-96 (ID-Solutions), IDEAL-32 (ID-Solutions) or any other open magnetic beads-based automated systems.
“Method for the detection and quantification of circulating DNA and uses” PATENTED TECHNOLOGY
Official publication numbers of applications “FR3061720” / “WO2018/127674”
IDXtract-MAG, circulating cell-free DNA (cfDNA) extraction and isolation kit based on magnetic beads
Technology | Magnetic beads |
Product references and formats | IDXtract-MAG-24 (24 preps of 4ml) IDXtract-MAG-100 (100 preps of 4ml) |
Matrix | Plasma from 1 to 8 ml |
Utilisation | Automated protocol |
Applications | NGS (Next-Generation Sequencing), dPCR (digital PCR), Real-Time PCR |
Duration of protocol | <2h for 24 preps |
Storage temperature | Ambient temperature |
Stability | 1 year |
Associated consumables
- IDWELL-32, deepwell plate 2mL (96 wells)
- IDTIP-32, magnetic rod’s tip (8 samples)
- IDWELL 24, deepwell plate 10mL (24 wells)
- IDTIPWELL-24, combi Tip comb/Deepwell 10mL (24 tips/wells)
- IDLYSE-24, lysis plate 25mL (24 wells)
Associated materials
Fig.1
Characteristics of IDXtract-MAG extraction kit.
Fig.2
Example of the cfDNA profile obtained after extraction of healthy plasma with the IDXtract-MAG kit.
Documentation on request to info@id-solutions.fr
