a multiplex kit for EGFR mutations detection in tumor DNA
The IDEGFR kit offers a highly sensitive multiplex digital PCR tool to detect and quantify activating or resistance mutations of EGFR (Epidermal Growth Factor Receptor) involved in non-small cell lung cancer (NSCLC). The highest number of detectable mutations allows a better patient stratification for targeted therapies using tyrosine kinase inhibitors.
Three optimized kits provide a multiplex solution ( ready-to-use) allows, for each sample, a simultaneous amplification in one PCR reaction :
- Activating mutations (L858R, L861Q and Del19 – 1st kit) (Ref: IDEGFR(s) SENSI-50),
- Mutations of resistance (T790M and C797S (cis/trans) – 2nd kit) to 1st and 3rd generation targeted therapies (Ref: IDEGFR(s) RESIST-50),
- Activating mutations or rare mutations (S768I, G719X and ins20 – 3rd kit) (Ref: IDEGFR(s) RARE-50).
This kit can be used on circulating cell-free DNA (cfDNA) extracted from plasma samples or DNA extracted from FFPE tissues.
It is compatible with naica® system.
|Reference and format||IDEGFR(s) SENSI-50 (50 preps)
IDEGFR(s) RESIST-50 (50 preps)
IDEGFR(s) RARE-50 (50 preps)
|Matrix||Circulating DNA extracted from plasma
Genomic DNA extracted from FFPE
|Duration of the protocol||2h30 (12 samples with Sapphire chips)|
|Application||Digital PCR (dPCR)|
Characteristics of IDEGFR (SENSI / RESIST / RARE) kit