a cfDNA extraction kit using silica column method
IDXtract is an extraction kit that integrate a calibrated positive control (TPC) and an internal extraction control (ICE).
IDXtract is an extraction kit of circulating cell-free DNA (cfDNA) optimized for use on biological samples such as blood plasma.
The kit contains a calibrated positive control (TPC) and an internal extraction control (ICE) that allows the qualification and the quantification of the extraction process and the standardization of the concentration for each sample. The kit is based on silica membrane columns, that allows reproducible recovery of high-quality cfDNA.
This kit concentrates the cfDNA in a low elution volume suitable for a wide range of applications, including real-time PCR, digital PCR (dPCR), next-generation sequencing (NGS) and genomic hybridization technologies.
“Method for the detection and quantification of circulating DNA and uses” PATENTED TECHNOLOGY
Official publication numbers of applications “FR3061720” / “WO2018/127674”
IDXtract, cell free DNA (cfDNA) or circulating DNA purification kit based on silica-column
|Product reference and format||IDXtract-50 (50 preps)|
|Matrix||Plasma from 1 to 8 ml|
|Applications||NGS (Next-Generation Sequencing), dPCR (digital PCR), Real-Time PCR|
|Duration of the protocol||<2h for 50 preparations|
|Storage temperature||Ambient temperature|
- IDVAC-24, vacuum manifold for processing of 24 silica membrane columns for cfDNA purification
- IDVAC VALVE-24, valves for handling different flow rates of columns on IDVAC-24 Vacuum Manifold
- IDVAC Mini Adapter, adapter for connecting the columns to IDVAC-24 vacuum manifold
- IDPUMP, vacuum pump for manual extractions
Characteristics of IDXtract kit
Example of the cfDNA profile obtained after extraction of healthy plasma with the IDXtract kit.
Quantification comparison of cfDNA obtained after extraction with IDXtract and a competitor Q. The IDXtract kit achieves an average efficiency of 100% compared to competitor Q.