cfDNA quantification kit by digital PCR
IDQUANTd is a ready-to-use kit for detection and normalized quantification of cell-free DNA (cfDNA) by digital PCR (dPCR). It allows for amplification of a target sequence of the human genome, present at the rate of one copy per haploid genome.
It is an absolute quantification duplex system that allows, for each sample, to simultaneously amplify the extracted circulating cell-free DNA (cfDNA) and an exogenous internal control (ICE).
Normalization with the ICE control can only be performed if the DNA has been extracted using the controls provided in the IDXtract and IDXtract-MAG extraction kits (for any other systems, please contact us). Using ICE, the extraction efficiency of the target for each sample could be evaluated which allows inter-test fluctuations standardization for the same donor overtime.
Calibration with ICE is validated with the circulating cell-free DNA (cfDNA) extracted with the IDXtract or IDXtract-MAG kits and can be used with other extraction solutions (validation required). IDQUANTd can also be used without ICE to detect and quantify cfDNA.
This kit is compatible with the following instruments: Digital Droplet PCR QX200 (Biorad) and Naica System Crystal digital PCR (Stilla). For other instruments, please contact us.
“Method for the detection and quantification of circulating DNA and uses” PATENTED TECHNOLOGY
Official publication numbers of applications “FR3061720” / “WO2018/127674”
IDQUANTd, circulating cell free DNA (cfDNA) detection and quantification kit using droplet digital PCR (dPCR)
|Reference and format||IDQUANTd(b)-50 (50 preps)
IDQUANTd(s)-50 (50 preps)
(b) Biorad (s) Stilla
|Matrix||Extracted DNA (for all other matrices, please contact us)|
|Applications||Digital PCR (dPCR)|
|Duration of protocol||dPCR 4h|
- IDXtract, silica-column based extraction and purification kit for circulating cell-free DNA
- IDXtract-MAG, magnetic beads-based extraction and purification kit for circulating cell-free DNA
- IDXtract-MAG-FFPE, magnetic beads-based extraction and purification kit for DNA from formaldehyde-fixed and paraffin-embedded tissue sections (FFPE)
Characteristics of IDQUANTd kit
Digital PCR data (dPCR) obtained with the kit IDQUANTd on circulating DNA extracted from healthy plasma with the kit IDXtract.
Plasmas were enriched with an increasing amount of DNA before extraction with the IDXtract kit. The analysis of the [cfDNA] / [ICE] ratio shows no difference between the repetitions and makes it possible to compare this ratio between the samples without taking into account the variations involved.