a multiplex kit for NRAS mutations detection in tumor DNA
The ready-to-use IDNRAS kit is a multiplexed and sensitive tool for the detection and quantification by digital PCR (dPCR) of NRAS‘s mutations (NRAS proto-oncogene, GTPase) in codons 12 and 13 of exon 2 and codon 61 of exon 3.
The NRAS gene, located on chromosome 1p13.2, encodes a 21.2 kDa GTPase that plays a key role in EGFR (Epidermal Growth Factor Receptor) signal transduction targeting downstream effectors in the signaling pathway. NRAS isoform is a part of the RAS protein family (KRAS, NRAS, HRAS). Mutations in NRAS account for about 11% for all RAS mutations in human tumors. Melanoma, acute myeloid leukemia and multiple myeloma have the highest mutation frequencies with rates of 94%, 59% and 45% respectively. NRAS mutations are rarely found in colorectal cancers (14%) and squamous cell carcinomas of the head and neck (5%). The most common mutations are found in codons 12 and 13 of exon 2 (35%) and codon 61 of exon 3 (63%) of NRAS gene.
This optimized kit provides a multiplex solution allowing, for each sample, to simultaneously amplify, in one PCR reaction, the NRAS‘s mutations at positions G12, G13 and Q61 (Ref: IDNRAS(b)-50, IDNRAS(s)-50).
This kit can be used on circulating cell-free DNA (cfDNA) extracted from plasma samples or DNA extracted from FFPE tissues.
It is compatible with the following instruments: Droplet Digital PCR QX200 (Biorad) and Naica System Crystal Digital PCR (Stilla). For other instruments, please contact us.
|Reference and format||IDNRAS(b)-50 (50 preps)
IDNRAS(s)-50 (50 preps)
(b) Biorad (s) Stilla
|Matrix||Circulating DNA extracted from plasma
Genomic DNA extracted from FFPE
|Application||Digital PCR (dPCR)|
|Duration of the protocol||4h (QX200-96 samples) and 2h (Naica-12 samples)|
- IDXtract, silica-column based extraction and purification kit for circulating DNA
- IDXtract-MAG, magnetic beads-based extraction and purification kit for circulating DNA
- IDXtract-MAG-FFPE, magnetic beads-based extraction and purification kit for DNA from formaldehyde-fixed and paraffin-embedded tissue sections (FFPE)
Characteristics of IDNRAS kit