a multiplex kit for the detection of BRAF gene duplication and mutations in tumor DNA
The ready-to-use IDBRAF-CNS kit is a multiplexed and sensitive tool for the detection and quantification by digital PCR (dPCR) of V600E mutation and the tyrosine kinase domain (TKD) duplication of the BRAF gene (B-Raf proto-oncogene, serine/threonine kinase).
TKD duplication of the BRAF gene, located at 7q34, systematically accompanies its fusion with the KIAA1549 gene (7q34). The detection of the KIAA1549-BRAF fusion is, according to the WHO recommendation (1), necessary for the classification of certain brain tumors in children and young adults.
The KIAA1549-BRAF fusion is a feature of pilocytic astrocytoma, but it has also been shown in rare cases of gangliogliomas, oligodendroglial tumors co-deleted for 1p / 19q.
BRAF V600E mutations are observed in many low-grade glioma subtypes, including 20% fibrillar astrocytomas, 50% gangliogliomas, 75% pleomorphic xanthoastrocytomas and 5% pilocytic astrocytomas.
This optimized kit provides a multiplex solution allowing, for each sample, to simultaneously amplify, in one PCR reaction, the V600E mutations (c.1799T>A) and the fusion of the KIAA1549-BRAF genes (Ref: IDBRAF-CNS(b)-25, IDBRAF-CNS(s)-25).
This kit can be used on circulating cell-free DNA (cfDNA) extracted from plasma samples or DNA extracted from FFPE tissues. For all other matrices, please contact us.
It is compatible with the following instruments: Digital Droplet PCR QX200 (Biorad) and Naica System Crystal Digital PCR (Stilla). For other instruments, please contact us.
(1) Louis, D.N., Ohgaki, H., Wiestler, O.D., Cavenee, W.K… WHO Classification of Tumours of the Central Nervous System, Revised. Fourth Edition T (2016)
IDBRAF-CNS, a tumor DNA detection kit for BRAF mutation analysis in pediatric cerebral cancer (targeting V600E (c.1799T>A) mutation and KIAA1549–BRAF fusion) / ctBRAF-CNS Mutation Detection Kit
|Reference and format||IDBRAF-CNS(b)-25 (25 preps)
IDBRAF-CNS(s)-25 (25 preps)
(b) Biorad (s) Stilla
|Matrix||Genomic DNA extracted from FFPE (for all other matrices, please contact us)|
|Application||Digital PCR (dPCR)|
|Duration of the protocol||4h (QX200-96 samples) and 2h (Naica-12 samples)|
- IDXtract, silica-column based extraction and purification kit for circulating DNA
- IDXtract-MAG, magnetic beads-based extraction and purification kit for circulating DNA
- IDXtract-MAG-FFPE, magnetic beads-based extraction and purification kit for DNA from formaldehyde-fixed and paraffin-embedded tissue sections (FFPE)
Characteristics of IDBRAF-CNS kit