a multiplex kit for BRAF mutations detection in tumor DNA
The ready-to-use IDBRAF kit is a multiplexed and sensitive tool for the detection and quantification by digital PCR (dPCR) of BRAF‘s mutations (B-Rad proto-oncogene, serine/threonine kinase) in exon 15.
The BRAF gene, located on chromosome 7q34, plays a major signal transduction role within the MAPK pathway. V600E mutation, which is the most common, accounts for more than 85% of BRAF mutations in metastatic melanoma, more than 50% in metastatic non-small cell lung carcinoma (NSCLC) and more than 11% in metastatic colon cancer. V600E mutation results in a permanent and unregulated activation of the MAPK pathway without any upstream extrinsic activating signal.
This optimized kit provides a multiplex solution allowing, for each sample, to simultaneously amplify, in one PCR reaction, the BRAF‘s mutations at positions L597, A598, V600 and K601, with a distinction of the V600E mutation (Ref: IDBRAF(b)-50, IDBRAF(s)-50).
This kit can be used on circulating cell-free DNA (cfDNA) extracted from plasma samples or DNA extracted from FFPE tissues.
It is compatible with the following instruments: Droplet Digital PCR QX200 (Biorad) and Naica System Crystal Digital PCR (Stilla). For other instruments, please contact us.
|Reference and format||IDBRAF(b)-50 (50 preps)
IDBRAF(s)-50 (50 preps)
(b) Biorad (s) Stilla
|Matrix||Circulating DNA extracted from plasma
Genomic DNA extracted from FFPE
|Application||Digital PCR (dPCR)|
|Duration of the protocol||4h (QX200-96 samples) and 2h (Naica-12 samples)|
- IDXtract, silica-column based extraction and purification kit for circulating DNA
- IDXtract-MAG, magnetic beads-based extraction and purification kit for circulating DNA
- IDXtract-MAG-FFPE, magnetic beads-based extraction and purification kit for DNA from formaldehyde-fixed and paraffin-embedded tissue sections (FFPE)
Characteristics of IDBRAF kit