IDXTRACT is an extraction kit of total circulating DNA (cfDNA) optimized for use on biological samples such as blood plasma.
The kit is based on silica membrane columns, allowing reproducible recovery of high quality cfDNA. It contains a calibrated Positive Control (TPC) and an Internal Extraction Control (ICE) allowing the qualification and the quantification of the extraction process and the standardization of the concentration for each sample.
This kit concentrates the total cfDNA in a small elution volume suitable for a wide range of applications, including real-time PCR, digital PCR (dPCR), next-generation sequencing (NGS) and genomic hybridization technologies.
“Method for the detection and quantification of circulating DNA and uses”
Official publication numbers of applications “FR3061720” / “WO2018/127674”
|Product reference and format||IDXtract-48 (48 preps)|
|Matrix||plasma from 1 to 8 ml|
|Utilisation||manual protocol with possible automating|
|Applications||NGS (Next-Generation Sequencing), dPCR (droplet digital PCR), Real-Time PCR|
|Duration of the protocol||<2h for 48 preparations|
|Storage temperature||ambient temperature|
Characteristics of IDXtract kit
Example of the cDNA profile obtained after extraction of healthy plasma with the IDXtract kit.
Quantification comparison of cDNA obtained after extraction with IDxtract and a competitor Q. The IDXtract kit achieves an average efficiency of 100% compared to competitor Q.