a multiplex kit for IDH1 and IDH2 mutations detection in tumor DNA
The ready-to-use IDIDH1-2 kit is a multiplexed and sensitive tool for the detection and quantification by digital PCR (dPCR) of mutations in the IDH1 (Isocitrate DeHydrogenase (NADP(+)) 1, cytosolic) and IDH2 (Isocitrate DeHydrogenase (NADP(+)) 2, mitochondrial) genes involved in the classification of brain tumors in adults.
Mutations in IDH1 gene, located at 2q34, and IDH2 gene, located at 15q26.1, allow to distinguish between the subtypes of “low-grade” gliomas and secondary glioblastomas.
This optimized kit provides a multiplex solution allowing, for each sample, to simultaneously amplify, in one PCR reaction, several variants in the both region of IDH1 (R132X – R132H, R132C, R132S, R132G, R132L) and IDH2 (R172X – R172W, R172G, R172K, R172M) (Ref: IDIDH1-2(b)-25, IDIDH1-2(s)-25).
This kit can be used on circulating cell-free DNA (cfDNA) extracted from plasma samples or DNA extracted from FFPE tissues.
It is compatible with the following instruments: Digital Droplet PCR QX200 (Biorad) and Naica System Crystal Digital PCR (Stilla). For other instruments, please contact us.
IDIDH1-2, a tumor DNA detection kit for IDH1 and IDH2 mutation analysis in cerebral cancer (targeting R132X and R172X mutations) / ctIDH1-2 Mutation Detection Kit
|Reference and format||IDIDH1-2(b)-25 (25 preps)
IDIDH1-2(s)-25 (25 preps)
(b) Biorad (s) Stilla
|Matrix||Circulating DNA extracted from plasma
Genomic DNA extracted from FFPE
|Application||Digital PCR (dPCR)|
|Duration of the protocol||4h (QX200-96 samples) and 2h (Naica-12 samples)|
- IDXtract, silica-column based extraction and purification kit for circulating DNA
- IDXtract-MAG, magnetic beads-based extraction and purification kit for circulating DNA
- IDXtract-MAG-FFPE, magnetic beads-based extraction and purification kit for DNA from formaldehyde-fixed and paraffin-embedded tissue sections (FFPE)
Characteristics of IDIDH1-2 kit