IDH3F3A

The IDH3F3A kit is a ready-to-use multiplex systemthat allows for each sample to detect and quantify the K27M*, G34R* and G34V*mutations of H3F3A (H3 histone family member 3A) in a single digital PCR reaction for the classification of brain tumours.

Molecular alterations of the H3F3A gene appear in many cancers, including brain cancers. These mutations cause a deregulation of DNA methylation. The most common mutations include single nucleotide polymorphisms K27M (high prevalence in young adults), G34R and G34V (high prevalence in adults).

*This numbering follows the histone nomenclature. However, these mutations can be referred to as K28M, G35R and G35V in some scientific publications

This kit can be used on circulating DNA and DNA isolated from formaldehyde-fixed and paraffin-embedded tissues (FFPE).

It is compatible with the following instruments: Digital droplet PCR QX200 (Biorad) and Naica system digital droplet PCR (Stilla). For others, please contact us.

This kit can be sold included in the IDPCTC panel.

Reference and formatIDH3F3A-25 (K27M, G34R, G34V) (25 preps)
IDPCTC panel (25 preps)
MatrixCirculating DNA extracted from plasma and FFPE
Application digital PCR (dPCR)
Duration of the protocol4h (QX200-96 samples) and 2h (Naica-12 samples)
Storage temperature-20°C
Stability1 year

Associated consumables

  • IDXtract, silica-based extraction and purification kit of total circulating DNA
  • IDXtract-MAG, magnetic beads-based extraction and purification kit of total circulating DNA
  • IDxtract-MAG-FFPE, DNA extraction and purification kit from formaldehyde-fixed and paraffin-embedded samples (FFPE)

Fig.1

Characteristics of IDH3F3A kit

Fig.2

Results obtained in digital PCR using IDH3F3A on synthetic DNA carrying mutations of H3F3A :

-K27M, G34R, G34V

 

Fig.3

The IDH3F3A kit has been validated for the detection of K27M, G34R and G34V mutations present on FFPE extracted DNA in comparison with NGS.

 

 

 

Documentation on request to info@id-solutions.fr