The IDH3F3A kit is a ready-to-use multiplex systemthat allows for each sample to detect and quantify the K27M*, G34R* and G34V*mutations of H3F3A (H3 histone family member 3A) in a single digital PCR reaction for the classification of brain tumours.
Molecular alterations of the H3F3A gene appear in many cancers, including brain cancers. These mutations cause a deregulation of DNA methylation. The most common mutations include single nucleotide polymorphisms K27M (high prevalence in young adults), G34R and G34V (high prevalence in adults).
*This numbering follows the histone nomenclature. However, these mutations can be referred to as K28M, G35R and G35V in some scientific publications
This kit can be used on circulating DNA and DNA isolated from formaldehyde-fixed and paraffin-embedded tissues (FFPE).
It is compatible with the following instruments: Digital droplet PCR QX200 (Biorad) and Naica system digital droplet PCR (Stilla). For others, please contact us.
This kit can be sold included in the IDPCTC panel.
|Reference and format||IDH3F3A-25 (K27M, G34R, G34V) (25 preps)
IDPCTC panel (25 preps)
|Matrix||Circulating DNA extracted from plasma and FFPE|
|Application||digital PCR (dPCR)|
|Duration of the protocol||4h (QX200-96 samples) and 2h (Naica-12 samples)|
Characteristics of IDH3F3A kit
Results obtained in digital PCR using IDH3F3A on synthetic DNA carrying mutations of H3F3A :
-K27M, G34R, G34V
The IDH3F3A kit has been validated for the detection of K27M, G34R and G34V mutations present on FFPE extracted DNA in comparison with NGS.