IDEGFR is a ready-to-use kit for the detection and quantification by digital PCR of EGFR (epidermal growth factor receptor) mutations involved in non-small cell lung cancer (NSCLC) patient stratification for targeted therapies using tyrosine kinase inhibitors and associated resistances.

This is a multiplexed quantification system that allows for each sample the simultaneous amplification in 3 reactions: activating mutations (L858R, L861Q and Del19 – 1st reaction), mutations of resistances (T790M and C797S – 2nd reaction) and rare mutations (S768I, G719X ans ins20 – 3rd reaction).

Each of the reactions can be sold separately.

This kit can be used on a circulating DNA extract or DNA extract isolated from FFPE tissues.

It is compatible with the following instruments: Digital droplet PCR QX200 (Biorad) and Naica digital system droplet PCR (Stilla). For others, contact us

Reference and formatIDEGFR-50 (50 preps)
IDEGFR-sensi (L858R/L861Q/del19) (50 preps)
IDEGFR-resist (T790M/C797S) (50 preps)
IDEGFR-rare (S768I,/G719X/ins20) (50 preps)
MatrixCirculating DNA extracted from plasma and FFPE
Application digital PCR (dPCR)
Duration of the protocol4h (QX200-96 samples) and 2h (Naica-12 samples)
Storage temperature-20°C
Stability1 year

Associated consumables

  • IDXtract, silica-based extraction and purification kit of total circulating DNA
  • IDXtract-MAG, magnetic beads-based extraction and purification kit of total circulating DNA
  • IDxtract-MAG-FFPE, DNA extraction and purification kit from formaldehyde-fixed and paraffin-embedded samples (FFPE)


Characteristics of IDEGFR kit



Results obtained in digital PCR using IDEGFR on synthetic DNA carrying mutations of EGFR :

L858R, L861Q and Del19

-T790M, C797S-cis / trans

-S768I, G719X, Ins20.



The IDEGFR kit has been validated for the detection of T790M, L858R and Del19 mutations present on circulating DNA in comparison with NGS analyzes and Cobas test CE-IVD (Roche Diagnostics US).





Documentation on request to