IDEGFR is a ready-to-use kit for the detection and quantification by digital PCR of EGFR (epidermal growth factor receptor) mutations involved in non-small cell lung cancer (NSCLC) patient stratification for targeted therapies using tyrosine kinase inhibitors and associated resistances.
This is a multiplexed quantification system that allows for each sample the simultaneous amplification in 3 reactions: activating mutations (L858R, L861Q and Del19 – 1st reaction), mutations of resistances (T790M and C797S – 2nd reaction) and rare mutations (S768I, G719X ans ins20 – 3rd reaction).
Each of the reactions can be sold separately.
This kit can be used on a circulating DNA extract or DNA extract isolated from FFPE tissues.
It is compatible with the following instruments: Digital droplet PCR QX200 (Biorad) and Naica digital system droplet PCR (Stilla). For others, contact us
|Reference and format||IDEGFR-50 (50 preps)
IDEGFR-sensi (L858R/L861Q/del19) (50 preps)
IDEGFR-resist (T790M/C797S) (50 preps)
IDEGFR-rare (S768I,/G719X/ins20) (50 preps)
|Matrix||Circulating DNA extracted from plasma and FFPE|
|Application||digital PCR (dPCR)|
|Duration of the protocol||4h (QX200-96 samples) and 2h (Naica-12 samples)|
Characteristics of IDEGFR kit
Results obtained in digital PCR using IDEGFR on synthetic DNA carrying mutations of EGFR :
–L858R, L861Q and Del19
-T790M, C797S-cis / trans
-S768I, G719X, Ins20.
The IDEGFR kit has been validated for the detection of T790M, L858R and Del19 mutations present on circulating DNA in comparison with NGS analyzes and Cobas test CE-IVD (Roche Diagnostics US).