The IDBRAF-CNS kit is a ready-to-use multiplex system that allows for each sample to detect and quantify the V600E (c.1799T>A) BRAF (B-Raf proto-oncogene, serine/threonine kinase) mutation and the KIAA1549-BRAF fusion in a single digital PCR reaction.
The BRAF fusion involving the KIAA1549 gene is a feature of pilocytic astrocytoma (represent the most common glioma subtype in young patients), but it has also been shown in rare cases of gangliogliomas, oligodendroglial tumors co-deleted for 1p / 19q, and is also a common feature of disseminated oligodendroglioma-like leptomeningeal neoplasm.
The BRAF V600E mutation has also been found in low grade gliomas in children. BRAF V600E mutations are observed in many low-grade glioma subtypes, including 20% fibrillar astrocytomas, 50% gangliogliomas, 75% pleomorphic xanthoastrocytomas and 5% pilocytic astrocytomas.
This kit can be used on circulating DNA and DNA isolated from formaldehyde-fixed and paraffin-embedded tissues (FFPE).
It is compatible with the following instruments: Digital droplet PCR QX200 (Biorad) and Naica system digital droplet PCR (Stilla). For others, please contact us.
This kit can be sold included in the IDPCTC panel.
|Reference and format||IDBRAF(b) CNS-25 or IDBRAF(s) CNS-25 (V600E, KIAA1549-BRAF) (25 preps)
IDPCTC panel (25 preps)
(b) Biorad (s) Stilla
|Matrix||Circulating DNA extracted from plasma and FFPE|
|Application||digital PCR (dPCR)|
|Duration of the protocol||4h (QX200-96 samples) and 2h (Naica-12 samples)|
Characteristics of IDBRAF kit
Results obtained in digital PCR using IDBRAF on synthetic DNA carrying mutations of BRAF :
The IDBRAF kit has been validated for the detection of V600E and KIAA-BRAF fusion present on FFPE extracted DNA in comparison with NGS.