IDBRAF-CNS

The IDBRAF-CNS kit is a ready-to-use multiplex system that allows for each sample to detect and quantify the V600E (c.1799T>A) BRAF (B-Raf proto-oncogene, serine/threonine kinase) mutation and the KIAA1549-BRAF fusion in a single digital PCR reaction.

The BRAF fusion involving the KIAA1549 gene is a feature of pilocytic astrocytoma (represent the most common glioma subtype in young patients), but it has also been shown in rare cases of gangliogliomas, oligodendroglial tumors co-deleted for 1p / 19q, and is also a common feature of disseminated oligodendroglioma-like leptomeningeal neoplasm.

The BRAF V600E mutation has also been found in low grade gliomas in children. BRAF V600E mutations are observed in many low-grade glioma subtypes, including 20% fibrillar astrocytomas, 50% gangliogliomas, 75% pleomorphic xanthoastrocytomas and 5% pilocytic astrocytomas.

This kit can be used on circulating DNA and DNA isolated from formaldehyde-fixed and paraffin-embedded tissues (FFPE).

It is compatible with the following instruments: Digital droplet PCR QX200 (Biorad) and Naica system digital droplet PCR (Stilla). For others, please contact us.

This kit can be sold included in the IDPCTC panel. 

Reference and formatIDBRAF(b) CNS-25 or IDBRAF(s) CNS-25 (V600E, KIAA1549-BRAF) (25 preps)
IDPCTC panel (25 preps)

(b) Biorad (s) Stilla
MatrixCirculating DNA extracted from plasma and FFPE
Application digital PCR (dPCR)
Duration of the protocol4h (QX200-96 samples) and 2h (Naica-12 samples)
Storage temperature-20°C
Stability1 year

Associated consumables

  • IDXtract, silica-based extraction and purification kit of total circulating DNA
  • IDXtract-MAG, magnetic beads-based extraction and purification kit of total circulating DNA
  • IDxtract-MAG-FFPE, DNA extraction and purification kit from formaldehyde-fixed and paraffin-embedded samples (FFPE)

Fig.1

Characteristics of IDBRAF kit

Fig.2

Results obtained in digital PCR using IDBRAF on synthetic DNA carrying mutations of BRAF :

-V600E

-KIAA-BRAF fusion

 

Fig.3

The IDBRAF kit has been validated for the detection of V600E and KIAA-BRAF fusion present on FFPE extracted DNA in comparison with NGS.

 

 

 

Documentation on request to info@id-solutions.fr